Full title

Solution structure of At5g39720.1 from Arabidopsis thaliana

Authors

• Betsy L. Lytle, Center for Fukaryotic Structural Genomics, US
• Francis C. Peterson, Center for Fukaryotic Structural Genomics, US
• Ejan M. Tyler, Center for Fukaryotic Structural Genomics, US
• Carrie L. Newman, Center for Fukaryotic Structural Genomics, US
• Dmitriy A. Vinarov, Center for Fukaryotic Structural Genomics, US
• John L. Markley, Center for Fukaryotic Structural Genomics, US
• Brian F. Volkman, Center for Fukaryotic Structural Genomics, US

Published

15 Jul 2024, 5:24 p.m.

Notes

Publication Abstract:

The three-dimensional structure of Arabidopsis thaliana protein At5g39720.1 was determined by NMR spectroscopy. It is the first representative structure of Pfam family PF06094, which contains protein sequences similar to that of AIG2, an A. thaliana protein of unknown function induced upon infection by the bacterial pathogen Pseudomonas syringae. The At5g39720.1 structure consists of a five-stranded beta-barrel surrounded by two alpha-helices and a small beta-sheet. A long flexible alpha-helix protrudes from the structure at the C-terminal end. A structural homology search revealed similarity to three members of Pfam family UPF0131. Conservation of residues in a hydrophilic cavity able to bind small ligands in UPF0131 proteins suggests that this may also serve as an active site in AIG2-like proteins.

Files

12 spectra, 0 peak lists, 0 additional files

Total HDD usage

1.4 GB

Uploaded by

Peter Güntert (guentert)

Entry type

Single protein molecule

Molecule identifier

AIG2-like protein A (UniProtKB AC Q9FIX2)

Components

• Polypeptide chain 1
  173 residues
  GHHHHHHLECSSDSLQLHNVFVYGSFQDPDVINVMLDRTPEIVSATLPGF
  QRFRLKGRLYPCIVPSEKGEVHGKVLMGVTSDELENLDAVEGNEYERVTV
  GIVREDNSEKMAVKTYMWINKADPDMFGEWNFEEWKRLHKKKFIETFKKI
  MECKKKPQGQGNDDISHVLREDQ

Source organism

Arabidopsis thaliana (TaxID 3702)

Expressed in

Escherichia coli (TaxID 562)

PDB ID

2G0Q

BMRB ID

7007

Publication

Lytle, B. L. et al. Solution structure ofArabidopsis thalianaprotein At5g39720.1, a member of the AIG2-like protein family. Acta Crystallographica Section F Structural Biology and Crystallization Communications 62, 490–493 (2006).
DOI: 10.1107/S1744309106015946